27 Why should the prepared plate be inverted during culture?

27 Why should the prepared plate be inverted during culture?

 

Petri dishes, petri plates

 

When it comes to inverting plates, have you really learned how to do it? As a newbie in scientific research, when I first entered the laboratory, the first skill I learned from my senior brothers and sisters was to invert the tablet. I believe that friends were confused as to why the plates should be cultivated upside down.

 

1 Prevent the culture medium from detaching from the petri dish

 

When we have just poured the plate and covered the petri dish, the culture medium is still warm and has just solidified. At this time, the water content is relatively large and the humidity is relatively high. On the lid of the dish, you will find some water droplets condensed on it. If the plate is placed upright, these condensed water droplets will flow into the culture medium, which not only easily causes contamination, but may also cause separation between the culture medium and the petri dish, causing the plate to easily slide when moving. In addition, if the moisture in the culture medium is large, the bacteria will flow around during inoculation and culture, causing the grown bacterial films to be connected together, making subsequent counting difficult.

 

 

2 petri plate is inverted for easy to take

 

During the culture process, we may need to open the incubator multiple times to pick up and place the plates. If the plates are placed in the right position, it is easy to accidentally lift the lid, increasing the risk of contamination. The inverted plate can maintain its original shape when taken, making it difficult for the bottom and lid to separate, thereby avoiding contamination by bacteria in the environment.

 

3 Conducive to bacterial growth and reproduction

 

Under culture conditions that require ventilation, an inverted plate can reduce the air flow on the surface of the culture medium and slow down the evaporation rate of water in the culture medium. This helps maintain moisture in solid media such as agar, keeping it elastic and prolonging the time it takes for the medium to dry out. Therefore, inverted culture is beneficial to the growth and reproduction of bacteria.

 

 

4 Prevent bacterial contamination during culture

 

When inverted culture, the air flow in the plate is reduced, which can reduce the infection of foreign bacteria as much as possible. This is essential to maintain the purity of the culture environment and avoid contamination of the medium and culture by miscellaneous bacteria.

 

5 Conducive to nutrient enrichment and observation and counting

 

When the plate is inverted for microbial culture, due to the influence of gravity, the nutrients in the culture medium will be concentrated on the surface of the culture medium and the sub-layer below the surface. This facilitates the growth and reproduction of microorganisms. At the same time, since the colonies do not spread too quickly, it is easier to identify the characteristics of the colonies during observation, which facilitates subsequent counting and analysis.

 

 

 

 

Manufacturer supplies,Factory direct sales, one piece is also the ex-factory price

 

Plastic petri dishes, various specifications, support customization, large quantity will give priority